Venezuelan Equine Encephalitis Virus

Testing Indications

The Venezuelan Equine Encephalitis serology testing is no longer available at the National Microbiology Laboratory. Other cross reactive flavivirus ELISA’s can be performed at NML and PHOL. Serological or molecular testing can only be performed based on relevant clinical information and after approval by the PHO microbiologist and may be referred to the CDC in Fort Collins.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Venezuelan Equine Encephalitis (VEE) serology

Whole blood or serum

5.0 ml blood or 1.0 ml serum

Clotted blood – vacutainer tubes (SST) 

Venezuelan Equine Encephalitis (VEE) serology


1.0 ml

Plasma with non-heparin anticoagulant (or serum)

Venezuelan Equine Encephalitis (VEE) serology


400 µl

Sterile container

Submission and Collection Notes


Molecular testing must must be preapproved by the PHO laboratory microbiologist. Contact PHO laboratory Customer Service at 416-235-6556 or 1877-604-4567 to request approval.


Complete the following on the General Test Requisition Form:

  • relevant travel history
  • onset date 
  • symptoms

Instructions for using SST tubes are found in the document titled: LAB-SD-008, Blood Collection using Serum Separator Tubes

Preparation Prior to Transport

Centrifuge if using SST. Place specimen in a biohazard bag and seal. Serology specimens should be stored at 2-8°C following collection and shipped to the PHOL on ice packs.

Specimens for molecular testing should be frozen and shipped on dry ice.

Special Instructions

Instructions for using SST tubes are found in the document titled: LAB-SD-008, Blood Collection Using Serum Separator Tubes.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

TAT is up to 28 days.


Results are reported to the ordering physician or health care provider as indicated on the requisition. Specimens that are positive for Venezuelan Equine Encephalitis are to be reported to the Medical Officer of Health as per Health Protection and Promotion Act.

Additional Information

All molecular testing must be preapproved by the PHOL microbiologist. Contact PHOL customer service at 416-235-6556 or 1-877-604-4567 to request approval.

Mis à jour le 20 juill. 2020